Agricultural Science

Agricultural Science

ISSN: 2291-4471 (Print)    ISSN: 2291-448X (Online)

Volume 1 (2013), No. 4, Pages 1-14

DOI: 10.12735/as.v1i4p01

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The Analysis of a Feed Component Imported into South Africa for Aflatoxin in Relation to Fungal and Mycotoxin Contamination

Mike F. Dutton1  G. Andersson2  E. V. Reiter3  E. Razzazi-Fazeli4  M. Mwanza1 

1Food, Environment and Health Research Group, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein, South Africa
2National Veterinary Institute, 751 89 Uppsala Sweden
3Department of Veterinary Public Health, Institute of Nutrition University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria and Institute of Animal Nutrition, Freie Universität Berlin, Königin-Luise-Str. 49, 14195 Berlin
4VetCore Facility for Research, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria

URL: https://doi.org/10.12735/as.v1i4p01

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Abstract

Currently there is concern with respect to the occurrence of mycotoxins in feed commodities, which could result in the loss of animal production and danger to consumers. Recent legislation to control the trading of such contaminated materials has been initiated with the result that it is imperative to be able to analyse for mycotoxins in feed commodities, rapidly and with sufficient accuracy to ensure that bulk cargoes of such materials are within set safety limits.

To this end a large batch (800 tonnes) of cotton-seed meal was consigned to a South African feed miller and was sampled according to a protocol devised under the European Union Framework 6 Biotracer programme. These were split and analysed for aflatoxins (AFs) by two laboratories using the VICAM fluorimetry aflatoxin method (VF) and by an high performance liquid chromatography (HPLC) method (HPLC) as part of another study to determine the statistical variation of using composite samples derived from a large bulk cargo (Reiter et al., 2011) .

The results from the HPLC method showed that all the composites were contaminated with aflatoxins (AF) ranging from 24 – 93μg/kg. A comparison of the two analytical methods used, showed that the results compared in terms of trend but in general the Vicam fluorimetry method (VF) gave a higher concentration of aflatoxin B1 (AFB1) ranging between 26 – 164μg/kg.

The levels of AF found were in several cases higher than those permitted by current legislation and would not have been allowed into the European Union. The methodology used allowed for reduced sampling and a more rapid method of analysis to assess AF contamination in commodities, subject to further development.

The predominant fungi isolated and identified were Aspergillus flavus and parasiticus, which are main producer of AFs in the environment.

Keywords: aflatoxin; cotton-seed meal; sampling; analysis; feed

To Cite this Article: Dutton, M. F., Andersson, G., Reiter, E. V., Razzazi-Fazeli, E., & Mwanza, M. (2013). The analysis of a feed component imported into South Africa for aflatoxin in relation to fungal and mycotoxin contamination. Agricultural Science, 1(4), 1-14. https://doi.org/10.12735/as.v1i4p01

Copyright © M. F. Dutton et al.

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This article is published under license to Science and Education Centre of North America. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License.

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The Analysis of a Feed Component Imported into South Africa for Aflatoxin in Relation to Fungal and Mycotoxin Contamination
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