Agricultural Science

Agricultural Science

ISSN: 2291-4471 (Print)    ISSN: 2291-448X (Online)

Volume 2 (2014), No. 4, Pages 35-43

DOI: 10.12735/as.v2i4p35

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Proteomic Responses of Wheat Roots during Enhanced Ultraviolet-B Radiation Stress

Duan Jiang-Yan1  Niu Wen-Ting1  Jia Zhen-Hu1 

1College of Life Science, Shanxi Normal University, Linfen 041004, P. R. China


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Enhanced ultraviolet-B (UV-B) radiation is one of the most important abiotic stresses that could influence the growth and physiological traits of plants. Wheat (Triticum aestivum) is also affected by present-day enhanced UV-B radiation. As protein metabolism plays an important role in plant adaptation to UV-B radiation, this study was designed to identify UV-B -responsive proteins in wheat roots. Wheat (Lin Fen NO.3) was exposed to UV-B radiation with normal light (group B) and only normal light (group CK) in growth chambers. Roots were harvested at 3d, 6d and 9d after UV-B radiation. Proteins were extracted and separated by two-dimensional gel electrophoresis (2-DE). 15 protein spots were regulated by UV-B radiation at 6d. Among these protein spots, 3 proteins were up-regulated and 10 proteins were down-regulated. 2 proteins were expressed only in CK group. 3 protein spots were identified using mass spectrometry. They were further verified as Ribulose-bisphosphate carboxylase activase; Catalase (CAT); Hypothetical protein. These data support the assumption that UV-B may have a regulatory role besides damaging effects and that an increased UV-B environment will likely increase this regulatory influence of UV-B radiation.

Keywords: wheat roots, Ultraviolet-B radiation, protein spot

To Cite this Article: Duan, J.-Y., Niu, W.-T., & Jia, Z.-H. (2014). Proteomic responses of wheat roots during enhanced Ultraviolet-B radiation stress. Agricultural Science, 2(4), 35-43.

Copyright © Duan Jiang-Yan et al.

Creative Commons License
This article is published under license to Science and Education Centre of North America. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License.

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Proteomic Responses of Wheat Roots during Enhanced Ultraviolet-B Radiation Stress